The Central Institute for Research on Buffaloes (Main Campus) is located at Hisar in Haryana state on the National Highway No.10 between latitude 29.10°N and longitude 75.46°E, about 170 Km north-west of the national capital - New Delhi. It is about 3 km from the Hisar Bus Stand on the Sirsa Road and about 5 km from the Hisar Railway Station. (Nearest airport is the Indira Gandhi International Airport, New Delhi for international flights and Palam Airport, New Delhi for domestic connections.)
It can be approached by road from New Delhi via National Highway No. 10, which proceeds onwards to Sirsa and Ferozepur towards western border of the country. National Highway No. 65 joins Hisar with Chandigarh, the capital of Haryana State in the north and various cities of Rajasthan towards the south. Buses for Hisar are available every 15 minutes from the Inter State Bus Terminus (ISBT) at New Delhi.
Hisar is on the railway map with broad gauze line from Delhi / New Delhi railway stations via Rewari - Rohtak - Bhiwani and onwards to some cities of Punjab such as Amritsar, Jalandhar, Ludhiana and Bhathinda on the other side. It is also connected to Jaipur and Bikaner in Rajasthan. However, there are no fast/long distance trains touching Hisar and the frequency of other train services is also less.

At the main campus, Main Lab.-cum-Administrative Building houses most of the laboratories of the three Divisions as well as different offices of the administration, including Director's office, ARIS cell, library, committee room and a seminar hall. Some laboratories and coordinating unit of the 'Network Project' are located in the Old Building. Ten well-designed animal sheds with adjoining open paddocks, sheds for research animals, feed and fodder stores, workshop and offices of some sections are located in the Old Campus. A Trainees' Hostel-cum-Guest House is available on campus for visiting scientists and officers. Various categories of staff quarters are also located on campus.

Ultra-modern animal shed with facilities for automatic feeding, cleaning, milking, heat detection and recording systems is being established at the main campus. Similarly, a modern Semen Freezing Laboratory is nearing completion of construction, which shall give a boost to frozen semen production at this centre in view of its large demand from the country and abroad. Similarly, Feed and Fodder Processing facilities are being strengthened.

The Sub-Campus is located at Bir Dosanjh about 8 km from Nabha town in Patiala District. It is off the National Highway No. 1 in Punjab, approx. 250 KM from Delhi. The nearest airport is at Chandigarh, 100 Km. from this Sub-Campus has newly constructed Administrative-Block in addition to the old Laboratory building housing library, semen processing, nutrition and genetics lab. Other available infrastructures include bull sheds, well-designed animal sheds, calving pen, feed stores and fodder processing unit, besides staff quarters and a guest house.

The Institute at the main campus has 1009 acres of land of which only 25% is arable. About 100 acres of salt affected land is being reclaimed in collaboration with CSSRI, Karnal. The sub-campus at Nabha has 588 acres of land. Broad details of land utilization at the two campuses are as under: